*/Disease Information(sort of like the Intro to your MiniResearch Writeup): Vibrio cholerae is a virulent bacteria that causes Cholera, a diarrheal infection. There are 1.4 to 4.3 cases of cholera a year and 28,000 to 142,000 deaths due to cholera a year. Cholera is spread mainly through water that has been contaminated by fecal matter. Outbreaks of cholera tend to be explosive due to the short incubation period of the bacteria (2 hours to 5 days).
-- -or link (or citation) to paper that contains assay information
-- links to assay reagents (substrates) pages.
--- List cost and quantity of substrate reagents, supplier, and catalog #
Structure (PDB or Homology model)
-- PDB # : 4LRQ
Current Inhibitors: 3[N-MORPHOLINO]PROPANE SULFONIC ACID
Expression Information (has it been expressed in bacterial cells): yes
Purification Method:
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MQKVLVVCMGNICRSPTAEAVLRAKAAQLKVDVEVDSAGTIGYHQGNPPDARSKAAGEKRGYSFSGIKARKIRDEDFVKFDWILAADQENLAELKARCPQSHQHKLSLMLSHSDSEYQEIPDPYYGGERGFELVLDLVEDAAEQFLLKLKQQGQH *length of your protein in Amino Acids
176 Molecular Weightof your protein in kiloDaltons using theExpasy ProtParamwebsite
*NCBI Gene # or RefSeq#: VC0395_A0559 VC395_1055
*Protein ID (NP or XP #) or Wolbachia#: 4LRQ
*Organism (including strain): Vibrio Cholerae
Etiologic Risk Group (see link below): 2
*/Disease Information(sort of like the Intro to your MiniResearch Writeup): Vibrio cholerae is a virulent bacteria that causes Cholera, a diarrheal infection. There are 1.4 to 4.3 cases of cholera a year and 28,000 to 142,000 deaths due to cholera a year. Cholera is spread mainly through water that has been contaminated by fecal matter. Outbreaks of cholera tend to be explosive due to the short incubation period of the bacteria (2 hours to 5 days).
Link to TDR Targets page (if present): N/A
Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)
http://www.ncbi.nlm.nih.gov/protein/487887718
Essentiality of this protein: Yes
Is it a monomer or multimer as biological unit? (make prediction at http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): 4 amino acid chains and 12 ligands in ASU
Complex of proteins?: yes
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
http://www.sciencedirect.com/science/article/pii/S1359644607001365
*EC#: 3.1.3.48
Link to BRENDA EC# page: http://www.brenda-enzymes.org/enzyme.php?ecno=3.1.3.48
-- Show screenshot of BRENDA enzyme mechanism schematic
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or othercompany) page for assay (see Sigma links below)
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/ptp101bul.pdf
-- -or link (or citation) to paper that contains assay information
-- links to assay reagents (substrates) pages.
--- List cost and quantity of substrate reagents, supplier, and catalog #
Structure (PDB or Homology model)
-- PDB # : 4LRQ
Current Inhibitors:
3[N-MORPHOLINO]PROPANE SULFONIC ACID
Expression Information (has it been expressed in bacterial cells): yes
Purification Method:
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MQKVLVVCMGNICRSPTAEAVLRAKAAQLKVDVEVDSAGTIGYHQGNPPDARSKAAGEKRGYSFSGIKARKIRDEDFVKFDWILAADQENLAELKARCPQSHQHKLSLMLSHSDSEYQEIPDPYYGGERGFELVLDLVEDAAEQFLLKLKQQGQH
*length of your protein in Amino Acids
176
Molecular Weightof your protein in kiloDaltons using theExpasy ProtParamwebsite
MolarExtinction coefficientof your protein at 280 nm wavelength:
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
*CDSGene Sequence(paste as text only):
*GC% Content for gene:
*CDS Gene Sequence (codon optimized) - copy fromoutputofPrimer DesignProtocol (paste as text only):
*GC% Content for gene (codon optimized):
Do Not Need this info for Spring (but still copy these lines to your Target page for now)
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
(link to DNA Works outputtext file-that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
**